IRI - Institut de Recherche Interdisciplinaire

Séminaire de Guillaume Brysbaert le 21 mai à 10h00 à l'IRI

guillaume — 2013-05-17T10:34:17Z

Identifying histone motifs on ISWI-ATPase

Guillaume Brysbaert
Interdisciplinary Research Institute
Villeneuve d'Ascq

Chromatin has a crucial role in eukaryotic cell regulation : it permits a regulation of genes expression which involves a lot of proteins of different natures. Beyond them, chromatin remodeling complexes hydrolyze ATP to slide, remove or replace some histones which build the nucleosome, the heart of chromatin. Here, I focus on ISWI chromatin remodelers and report results of sequence alignments of ISWI ATPase subunits selected from three species, S. cerevisiae, D. melanogaster and H. sapiens, with complete histone sequences (H1, H2A, H2B, H3, H4). Our aim was to search for sequence motifs relevant for (auto)-regulatory interactions in ISWI family members. The motif search revealed the presence of histonelike motifs from all histone variants on the catalytic unit of ISWI remodelers. We have determined tertiary structure of the motifs to test their accessibility to regulators. I discuss implications of the major motifs found for H4 and H2A on the regulation of ISWI, and relate these to the recent discoveries on ISWI.


Guillaume Brysbaert 2013/05/21

96200. Séminaire de Riccardo Peruzzini le 14 mai à 10h00 à l'IRI

guillaume — 2013-05-13T12:24:45Z

Molecular mechanisms of protein trapping and maturation in the Inter Membrane Space (IMS) of Mitochondria

Riccardo Peruzzini
Magnetic Resonance Center (CERM)
Florence, Italy


Riccardo Peruzzini 2013/05/13

Supplementary data

Emanuele — 2013-05-03T13:43:34Z

To retrieve data files of ChIp-Seq of Fioravanti et al., 2013, PLOS genetics, please fill-in the form below :

Description

guillaume — 2013-05-03T12:39:22Z

en construction - 10. Description

96300. Séminaire de Benoît Odaert le 30 avril à 10h00 à l'IRI

guillaume — 2013-04-22T14:19:59Z

An exposé on three lipid-protein interaction studies by NMR

Benoît Odaert
Institut de Chimie et Biologie des Membranes et Nano-objets
Pessac, France


Benoît Odaert 2013/04/30

Nouvel article

alexis — 2013-04-22T13:19:35Z

2011

Carette X, Blondiaux N, Willery E, Hoos S, Lecat-Guillet N, Lens Z, Wohlkönig A, Wintjens R, Soror SH, Frénois F, Dirié B, Villeret V, England P, Lippens G, Deprez B, Locht C, Willand N, Baulard AR.

Structural activation of the transcriptional repressor EthR from Mycobacterium tuberculosis by single amino acid change mimicking natural and synthetic ligands. Nucleic Acids Res. 2011 Dec 9

Flipo M, Desroses M, Lecat-Guillet N, Villemagne B, Blondiaux N, Leroux F, Piveteau C, Mathys V, Flament MP, Siepmann J, Villeret V, Wohlkönig A, Wintjens R, Soror SH, Christophe T, Jeon HK, Locht C, Brodin P, Déprez B, Baulard AR, Willand N.
Ethionamide Boosters. 2. Combining Bioisosteric Replacement and Structure-Based Drug Design To Solve Pharmacokinetic Issues in a Series of Potent 1,2,4-Oxadiazole EthR Inhibitors. J Med Chem. 2011 Dec 6.

A. Fréville, I. Landrieu, M. Adelaida Garcia-Gimeno, J. Vicogne, M. Montbarbon, B. Bertin, A. Verger, H. Kalamou, P. Sanz, E. Werkmeister, C. Pierrot and J. Khalife
Plasmodium falciparum inhibitor 3 homolog increases protein phosphatase type 1 activity and is essential for parasitic survival. J. Biol. Chem. November 28, 2011 as doi:10.1074/jbc.M111.276865.

K. Verreman, J.L. Baert, A. Verger, H. Drobecq, E. Ferreira, Y. de Launoit and D. Monté
The Coactivator Activator CoAA regulates PEA3 group member transcriptional activity. Biochem. J. 2011 ; 439 (3) : 469-77.

Lens Z., Dewitte F., Van Lint C., de Launoit Y., Villeret V. & Verger A.
Purification of Sumo-1 modified I kappa B alpha and complex formation with NF-kappa B. Protein expression and purification 2011 ; 80 (2) : 211-216.

Delattre AS, Saint N, Clantin B, Willery E, Lippens G, Locht C, Villeret V, Jacob-Dubuisson F.
Substrate recognition by the POTRA domains of TpsB transporter FhaC. Mol Microbiol 2011 ; 81 (1) : 99-112.

Merlin J, Stechly L, de Beaucé S, Monté D, Leteurtre E, van Seuningen I, Huet G, Pigny P.
Galectin-3 regulates MUC1 and EGFR cellular distribution and EGFR downstream pathways in pancreatic cancer cells. Oncogene 2011 ; 30 (22) : 2514-25.

Flipo M, Desroses M, Lecat-Guillet N, Dirie B, Carette X, Leroux F, Piveteau C, Demirkaya F, Lens Z, Rucktooa P, Villeret V, Christophe T, Jeon HK, Locht C, Brodin P, Deprez BP, Baulard A, Willand N.
Ethionamide boosters : Synthesis, Biological Activity and Structure-Activity Relationships of a series of 1,2,4-oxadiazole EthR inhibitors. J Med Chem. 2011 Apr 28 ; 54 (8) : 2994-3010.

Bontems F, Verger A, Dewitte F, Lens Z, Baert JL, Ferreira E, de Launoit Y, Sizun C, Guittet E, Villeret V, Monté D.
NMR structure of the human Mediator MED25 ACID domain. Journal of Structural Biology 2011 ; 174 : 245-251.

Offres d'emploi

guillaume — 2013-04-08T12:49:47Z

Applications are invited for a Postdoctoral position, immediately available with initial funding for 2 years. The successful candidates will join the CNRS (French Research Agency)-Interdisciplinary Research Institute, located in Lille, North of France. The project is devoted to structure resolution by NMR spectroscopy of protein(s) involved in transcriptional regulation. A full set of spectra has already been acquired, ensuring the feasibility of the project. The candidate should be motivated to join a multidisciplinary environment within the group and participate to a large number of collaborations. The ideal candidate holds a PhD in chemistry or biochemistry and has a strong background in protein resolution by NMR spectroscopy, using the latest developments in this area. Experience in molecular docking based on experimental data will be considered positively. The candidate should be able to work independently and participate creatively in project directions. The candidate must have good oral and written communication skills.

The position has no limitation on nationality. The CNRS seeks to increase the number of women in those areas where they are underrepresented and therefore explicitly encourages women to apply.

Applicants should provide a CV to isabelle.landrieu [At] univ-lille1.fr and vincent.villeret [At] iri.univ-lille1.fr

Yolcu Hassan

guillaume — 2013-04-02T15:23:11Z

Equipe : Nanobiointerfaces

tel : +33 3 62 53 17 41

Wang Qi

guillaume — 2013-04-02T15:20:56Z

Equipe : Nanobiointerfaces

tel : +33 3 62 53 17 23

Turcheniuk Konstantine

guillaume — 2013-04-02T15:17:45Z

Equipe : Nanobiointerfaces

tel : +33 3 62 53 17 27